분자생물학적 기법을 이용한 악골 골수염 병소의 세균 동정
Molecular Identification of Bacteria from Osteomyelitis of the Jaws
김미성, 김수관, 김생곤, 정해만,
소속 상세정보
김미성 ( ) - 조선대학교 치과대학 구강악안면외과학교실,
김수관 ( ) - 조선대학교 치과대학 구강악안면외과학교실,
김생곤 ( ) - 조선대학교 치과대학 인체생물학
정해만 ( ) - 조선대학교 치과대학 인체생물학교실
KMID : 0355620030290010048
Abstract
The purpose of this study was to isolate and identify the bacteria in osteomyelitis lesion of 3 patients. Two lesions were due to the post-infection after extraction. The other was resulted from mal-fixation of both sides of mandibular angles. Pus samples were collected by needle aspiration from the lesion and examined by culture method. Bacterial culture was performed in three culture systems (anaerobic, CO2, and aerobic incubator). Identification of the bacteria was performed by 16S rRNA gene cloning and nucleotide sequencing method. Our results showed that Streptococci species was predominantly isolated in both lesions of extraction socket. Only one species (Proteus vulagris) was detected in lesion of mandibular angle. This study was not sufficient to identify the causative bacteria in those osteomyelitis. However, our data may be offered the clue to solve the problem.
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